Differential Gene Transcription of Extracellular Matrix Components in Response to In Vivo Corneal Crosslinking (CXL) in Rabbit Corneas.

ABSTRACT:

Purpose

We studied changes in gene transcription after corneal crosslinking (CXL) in the rabbit cornea in vivo and identified potential molecular signaling pathways.

Methods

A total of 15 corneas of eight male New-Zealand-White rabbits were de-epithelialized and equally divided into five groups. Group 1 served as an untreated control. Groups 2 to 5 were soaked with 0.1% riboflavin for 20 minutes, which in Groups 3 to 5 was followed by UV-A irradiation at a fluence of 5.4 J/cm². Ultraviolet A (UVA) irradiation was delivered at 3 mW/cm² for 30 minutes (Group 3, standard CXL protocol), 9 mW/cm² for 10 minutes (Group 4, accelerated), and 18 mW/cm² for 5 minutes (Group 5, accelerated). At 1 week after treatment, corneal buttons were obt

SUBMITTER: Kling S 

PROVIDER: S-EPMC5727938 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature