A unique microenvironment in the developing liver supports the expansion of megakaryocyte progenitors.

ABSTRACT: The fetal liver is the site of a major expansion of the hematopoietic stem cell (HSC) pool and is also a privileged organ to study megakaryocyte progenitor differentiation. We identified in the mouse fetal liver at day 13.5 a discrete stromal cell population harboring a CD45^-TER119^-CD31^-CD51⁺VCAM-1⁺PDGFRα^- (V⁺P^-) phenotype that lacked colony-forming unit fibroblast activity and harbored an hepatocyte progenitor signature. This previously undescribed V⁺P^- population efficiently supported megakaryocyte production from mouse bone marrow HSC and human peripheral blood HSC-myeloid progenitors cultured in the presence of limited cytokine concentrations. Megakaryocytes obtained in V⁺

SUBMITTER: Brouard N 

PROVIDER: S-EPMC5728093 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature