Shrimp ATP synthase genes complement yeast null mutants for ATP hydrolysis but not synthesis activity.

ABSTRACT: The aim of this study was to examine the feasibility of employing a yeast functional complementation assay for shrimp genes by using the shrimp mitochondrial F(1)F(0)-ATP synthase enzyme complex as a model. Yeast mutants defective in this complex are typically respiratory-deficient and cannot grow on non-fermentable carbon sources such as glycerol, allowing easy verification of functional complementation by yeast growth on media with them as the only carbon source. We cloned the previous published sequence of ATP2 (coding for ATP synthase β subunit) from the Pacific white shrimp Penaeus vannamei (Pv) and also successfully amplified a novel PvATP3 (coding for the ATP synthase γ subunit). Analysis of the putative amino acid sequence of PvATP3 revealed a significant homology with the ATP synt

SUBMITTER: Wuthisathid K 

PROVIDER: S-EPMC5728117 | biostudies-literature | 2012 Oct

REPOSITORIES: biostudies-literature