Development of a tissue-specific ribosome profiling approach in Drosophila enables genome-wide evaluation of translational adaptations.

ABSTRACT: Recent advances in next-generation sequencing approaches have revolutionized our understanding of transcriptional expression in diverse systems. However, measurements of transcription do not necessarily reflect gene translation, the process of ultimate importance in understanding cellular function. To circumvent this limitation, biochemical tagging of ribosome subunits to isolate ribosome-associated mRNA has been developed. However, this approach, called TRAP, lacks quantitative resolution compared to a superior technology, ribosome profiling. Here, we report the development of an optimized ribosome profiling approach in Drosophila. We first demonstrate successful ribosome profiling from a specific tissue, larval muscle, with enhanced resolution compared to conventional TRAP approaches. We

SUBMITTER: Chen X 

PROVIDER: S-EPMC5728580 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature