Selecting the correct cellular model for assessing of the biological response of collagen-based biomaterials.

ABSTRACT: Accurate evaluation of the biological performance of biomaterials requires the correct assessment of their native-like cell ligation properties. However, cell attachment studies often overlook the details of the substrate-cell binding mechanisms, be they integrin-mediated or non-specific, and ignore the class- and species-specificities of the cell adhesion receptor involved. In this work we have used different collagen (Col) substrates (fibrillar collagens I, II and III and network-forming Col IV), containing different affinity cell-recognition motifs, to establish the influence of the receptor identity and species-specificity on collagen-cell interactive properties. Receptor expression was varied by using cells of different origin, or transfecting collagen-binding integrins into integrin-

SUBMITTER: Davidenko N 

PROVIDER: S-EPMC5729022 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature