Mapping platinum adducts on yeast ribosomal RNA using high-throughput sequencing.

ABSTRACT: Methods to map small-molecule binding sites on cellular RNAs are important for understanding interactions with both endogenous and exogenous compounds. Pt(ii) reagents are well-known DNA and RNA crosslinking agents, but sequence-specific and genome-wide identification of Pt targets following in-cell treatment is challenging. Here we describe application of high-throughput 'Pt-Seq' to identify Pt-rRNA adducts following treatment of S. cerevisiae with cisplatin.

SUBMITTER: Plakos K 

PROVIDER: S-EPMC5729026 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature