Locus-specific hypomethylation of the mouse IAP retrotransposon is associated with transcription factor-binding sites.

ABSTRACT:

Background

Intracisternal A particle (IAP) is one of the most transpositionally active retrotransposons in the mouse genome, but its expression varies between cell types. This variation is believed to arise from differences in the epigenetic state (e.g., DNA methylation) of the 5' long terminal repeat (LTR), where transcription starts. However, owing to the high copy number and high sequence similarity between copies, it is difficult to analyze the epigenetic states of individual IAP LTRs in a comprehensive manner.

Results

We have developed a method called Target Enrichment after Post-Bisulfite Adaptor Tagging (TEPBAT) to analyze the DNA methylation states of a large number of individual retrotransposon copies at once. Using this method, we determined the DNA methylation lev

SUBMITTER: Shimosuga KI 

PROVIDER: S-EPMC5729234 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature