Shigella depends on SepA to destabilize the intestinal epithelial integrity via cofilin activation.

ABSTRACT: Shigella is unique among enteric pathogens, as it invades colonic epithelia through the basolateral pole. Therefore, it has evolved the ability to breach the intestinal epithelial barrier to deploy an arsenal of effector proteins, which permits bacterial invasion and leads to a severe inflammatory response. However, the mechanisms used by Shigella to regulate epithelial barrier permeability remain unknown. To address this question, we used both an intestinal polarized model and a human ex-vivo model to further characterize the early events of host-bacteria interactions. Our results showed that secreted Serine Protease A (SepA), which belongs to the serine protease autotransporter of Enterobacteriaceae family, is responsible for critically disrupting the intestinal epithelial barrier. Such

SUBMITTER: Maldonado-Contreras A 

PROVIDER: S-EPMC5730386 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature