Nonreceptor tyrosine kinases ITK and BTK negatively regulate mast cell proinflammatory responses to lipopolysaccharide.

ABSTRACT:

Background

Mast cells are indispensable for LPS-induced septic hypothermia, in which TNF-α plays an essential role to initiate septic responses. ITK and BTK regulate mast cell responses to allergens, but their roles in mast cell responses in LPS-induced sepsis are unclear.

Objective

We sought to investigate the roles of ITK and BTK in mast cell responses during LPS-induced septic inflammation.

Methods

Mice (genetically modified or bone marrow-derived mast cell-reconstituted Sash) were given LPS to induce septic hypothermia in the presence or absence of indicated inhibitors. Flow cytometry was used to determine LPS-induced cell influx and TNF-α production in peritoneal cells. Microarray was used for genomewide gene expression analysis on bone marrow-derived mast cells.

SUBMITTER: Huang W 

PROVIDER: S-EPMC5730405 | biostudies-literature | 2016 Apr

REPOSITORIES: biostudies-literature