Efficient transgenesis and annotated genome sequence of the regenerative flatworm model Macrostomum lignano.

ABSTRACT: Regeneration-capable flatworms are informative research models to study the mechanisms of stem cell regulation, regeneration, and tissue patterning. However, the lack of transgenesis methods considerably hampers their wider use. Here we report development of a transgenesis method for Macrostomum lignano, a basal flatworm with excellent regeneration capacity. We demonstrate that microinjection of DNA constructs into fertilized one-cell stage eggs, followed by a low dose of irradiation, frequently results in random integration of the transgene in the genome and its stable transmission through the germline. To facilitate selection of promoter regions for transgenic reporters, we assembled and annotated the M. lignano genome, including genome-wide mapping of transcription start regions, and sh

SUBMITTER: Wudarski J 

PROVIDER: S-EPMC5730564 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature