Viperin Targets Flavivirus Virulence by Inducing Assembly of Noninfectious Capsid Particles.

ABSTRACT: Efficient antiviral immunity requires interference with virus replication at multiple layers targeting diverse steps in the viral life cycle. We describe here a novel flavivirus inhibition mechanism that results in interferon-mediated obstruction of tick-borne encephalitis virus particle assembly and involves release of malfunctioning membrane-associated capsid (C) particles. This mechanism is controlled by the activity of the interferon-induced protein viperin, a broad-spectrum antiviral interferon-stimulated gene. Through analysis of the viperin-interactome, we identified the Golgi brefeldin A-resistant guanine nucleotide exchange factor 1 (GBF1) as the cellular protein targeted by viperin. Viperin-induced antiviral activity, as well as C-particle release, was stimulated by GBF1 inhibiti

SUBMITTER: Vonderstein K 

PROVIDER: S-EPMC5730767 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature