Mechanisms of HERV-K (HML-2) Transcription during Human Mammary Epithelial Cell Transformation.

ABSTRACT: Increasing evidence suggests that repetitive elements may play a role in host gene regulation, particularly through the donation of alternative promoters, enhancers, splice sites, and termination signals. Elevated transcript expression of the endogenous retrovirus group HERV-K (HML-2) is seen in many human cancers, although the identities of the individual proviral loci contributing to this expression as well as their mechanisms of activation have been unclear. Using high-throughput next-generation sequencing techniques optimized for the capture of HML-2 expression, we characterized the HML-2 transcriptome and means of activation in an in vitro model of human mammary epithelial cell transformation. Our analysis showed significant expression originating from 15 HML-2 full-length proviruses,

SUBMITTER: Montesion M 

PROVIDER: S-EPMC5730778 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature