Single-cell RNA sequencing reveals developmental heterogeneity among early lymphoid progenitors.

ABSTRACT: Single-cell RNA sequencing is a powerful technology for assessing heterogeneity within defined cell populations. Here, we describe the heterogeneity of a B220⁺CD117^intCD19^-NK1.1^- uncommitted hematopoietic progenitor having combined lymphoid and myeloid potential. Phenotypic and functional assays revealed four subpopulations within the progenitor with distinct lineage developmental potentials. Among them, the Ly6D⁺SiglecH^-CD11c^- fraction was lymphoid-restricted exhibiting strong B-cell potential, whereas the Ly6D^-SiglecH^-CD11c^- fraction showed mixed lympho-myeloid potential. Single-cell RNA sequencing of these subsets revealed that the latter population comprised a mixture of cells

SUBMITTER: Alberti-Servera L 

PROVIDER: S-EPMC5730887 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature