Cell cycle-related metabolism and mitochondrial dynamics in a replication-competent pancreatic beta-cell line.

ABSTRACT: Cell replication is a fundamental attribute of growth and repair in multicellular organisms. Pancreatic beta-cells in adults rarely enter cell cycle, hindering the capacity for regeneration in diabetes. Efforts to drive beta-cells into cell cycle have so far largely focused on regulatory molecules such as cyclins and cyclin-dependent kinases (CDKs). Investigations in cancer biology have uncovered that adaptive changes in metabolism, the mitochondrial network, and cellular Ca²⁺ are critical for permitting cells to progress through the cell cycle. Here, we investigated these parameters in the replication-competent beta-cell line INS 832/13. Cell cycle synchronization of this line permitted evaluation of cell metabolism, mitochondrial network, and cellular Ca²⁺ compartme

SUBMITTER: Montemurro C 

PROVIDER: S-EPMC5731404 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature