Post-irradiation promotes susceptibility to reprogramming to pluripotent state in human fibroblasts.

ABSTRACT: Ionizing radiation causes not only targeted effects in cells that have been directly irradiated but also non-targeted effects in several cell generations after initial exposure. Recent studies suggest that radiation can enrich for a population of stem cells, derived from differentiated cells, through cellular reprogramming. Here, we elucidate the effect of irradiation on reprogramming, subjected to two different responses, using an induced pluripotent stem cell (iPSC) model. iPSCs were generated from non-irradiated cells, directly-irradiated cells, or cells subsequently generated after initial radiation exposure. We found that direct irradiation negatively affected iPSC induction in a dose-dependent manner. However, in the post-irradiated group, after five subsequent generations, cells bec

SUBMITTER: Lee SB 

PROVIDER: S-EPMC5731415 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature