Multilocus genotyping of Giardia duodenalis isolates from breeding cattery cats in Japan.

ABSTRACT:

Objectives

The present study reports the multilocus genotyping of Giardia duodenalis isolates from cats maintained in breeding catteries in Japan and discusses their potential for zoonotic transmission.

Methods

A total of 41 faecal samples positive for Giardia-specific antigen were procured from cats maintained in five breeding catteries and subjected to PCR to amplify four gene loci, namely small subunit ribosomal RNA (SSU rRNA), glutamate dehydrogenase (gdh), beta-giardin (bg) and triose phosphate isomerase (tpi ). The PCR-amplified DNA fragments were sequenced to determine the G duodenalis genotypes (synonym for assemblages).

Results

The most commonly occurring single assemblage was assemblage F (68.3%; n = 28/41), f

SUBMITTER: Ito Y 

PROVIDER: S-EPMC5731622 | biostudies-literature | 2017 Jul-Dec

REPOSITORIES: biostudies-literature