Data-driven programmatic approach to analysis of basophil activation tests.

ABSTRACT:

Background

Conventional data analysis of flow cytometry-based basophil activation testing requires repetitive, labor-intensive analysis that hampers efforts to standardize testing for clinical applications. Using an open-source platform, we developed and implemented a programmatic approach to the analysis of the basophil activation test (BAT) by flow cytometry.

Methods

Using the BÜHLMANN FlowCAST® assay, peripheral blood from peanut allergic patients undergoing oral immunotherapy was incubated with peanut allergens (Arah1, Arah2, Arah6, whole peanut extract) and stained with fluorescent antibodies to CCR3 and CD63 for the development of a data-driven programmatic analysis using Bioconductor and R. Basophil identification using clustering and classification was validated usin

SUBMITTER: Patil SU 

PROVIDER: S-EPMC5732088 | biostudies-literature | 2018 Jul

REPOSITORIES: biostudies-literature