High-Content Monitoring of Drug Effects in a 3D Spheroid Model.

ABSTRACT: A recent decline in the discovery of novel medications challenges the widespread use of 2D monolayer cell assays in the drug discovery process. As a result, the need for more appropriate cellular models of human physiology and disease has renewed the interest in spheroid 3D culture as a pertinent model for drug screening. However, despite technological progress that has significantly simplified spheroid production and analysis, the seeming complexity of the 3D approach has delayed its adoption in many laboratories. The present report demonstrates that the use of a spheroid model may be straightforward and can provide information that is not directly available with a standard 2D approach. We describe a cost-efficient method that allows for the production of an array of uniform spheroids, th

SUBMITTER: Mittler F 

PROVIDER: S-EPMC5732143 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature