Untargeted Metabolomics Approach in Halophiles: Understanding the Biodeterioration Process of Building Materials.

ABSTRACT: The aim of the study was to explore the halophile metabolome in building materials using untargeted metabolomics which allows for broad metabolome coverage. For this reason, we used high-performance liquid chromatography interfaced to high-resolution mass spectrometry (HPLC/HRMS). As an alternative to standard microscopy techniques, we introduced pioneering Coherent Anti-stokes Raman Scattering Microscopy (CARS) to non-invasively visualize microbial cells. Brick samples saturated with salt solution (KCl, NaCl (two salinity levels), MgSO₄, Mg(NO₃)₂), were inoculated with the mixture of preselected halophilic microorganisms, i.e., bacteria: Halobacillus styriensis, Halobacillus naozhouensis, Halobacillus hunanensis, Staphylococcus succinus, Marinococcus ha

SUBMITTER: Adamiak J 

PROVIDER: S-EPMC5732225 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature