Dermatophilus congolensis infection in sheep and goats in Delta region of Tamil Nadu.

ABSTRACT:

Aim

The study was conducted to isolate and identify Dermatophilus congolensis (DC) using conventional and molecular diagnostic techniques in scab materials collected from skin infections of sheep and goats in the Delta region of Tamil Nadu.

Materials and methods

A total of 20 scab samples collected from 18 goats and 2 sheep from Nagapattinam, Thanjavur, and Tiruvarur districts of Tamil Nadu. Smears were made from softened scab materials and stained by either Gram's or Giemsa staining. Isolation was attempted on blood agar plates, and colonies were stained by Gram's staining for morphological identification. Identification was also done by biochemical tests and confirmed by 16S rRNA polymerase chain reaction (PCR), followed by sequencing and phylogenetic analysis of th

SUBMITTER: Chitra MA 

PROVIDER: S-EPMC5732335 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature