Application of loop-mediated isothermal amplification assay in the detection of herpesvirus of turkey (FC 126 strain) from chicken samples in Nigeria.

ABSTRACT:

Aim

This study was designed to optimize and apply the use of loop-mediated isothermal amplification (LAMP) as an alternative to conventional polymerase chain reaction (PCR) for the detection of herpesvirus of turkeys (HVT) (FC 126 strain) in vaccinated and non-vaccinated poultry in Nigeria.

Materials and methods

HVT positive control (vaccine) was used for optimization of LAMP using six primers that target the HVT070 gene sequence of the virus. These primers can differentiate HVT, a Marek's disease virus (MDV) serotype 3 from MDV serotypes 1 and 2. Samples were collected from clinical cases of Marek's disease (MD) in chickens, processed and subjected to LAMP and PCR.

Results

LAMP assay for HVT was optimized. HVT was detected in 60% (3/5) and 100% (5/5) of the samples a

SUBMITTER: Adedeji AJ 

PROVIDER: S-EPMC5732347 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature