Comprehensive analysis of differentially expressed genes associated with PLK1 in bladder cancer.

ABSTRACT:

Background

The significance of PLK1 (polo-like kinase 1) has become increasingly essential as both a biomarker and a target for cancer treatment. Here, we aimed to determine the downstream genes of PLK1 and their effects on the carcinogenesis and progression of bladder cancer.

Methods

Specific siRNA was utilized to silence the target gene expression. The cell proliferation, invasion and migration of bladder cancer cells by MTT assay, BrdU assay and transwell assay. The differential expression genes were identified using Affymetrix HTA2.0 Array. The KEGG, GO and STRING analysis were used to analyze the signaling pathway and protein-protein interaction. Spearman analysis was used to analyze the correlation between protein and protein, between protein and clincopathologic chara

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC5732388 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature