The function and evolutionary significance of a triplicated Na,K-ATPase gene in a toxin-specialized insect.

ABSTRACT:

Background

The Na,K-ATPase is a vital animal cell-membrane protein that maintains the cell's resting potential, among other functions. Cardenolides, a group of potent plant toxins, bind to and inhibit this pump. The gene encoding the α-subunit of the pump has undergone duplication events in some insect species known to feed on plants containing cardenolides. Here we test the function of these duplicated gene copies in the cardenolide-adapted milkweed bug, Oncopeltus fasciatus, which has three known copies of the gene: α1A, α1B and α1C.

Results

Using RT-qPCR analyses we demonstrate that the α1C is highly expressed in neural tissue, where the pump is generally thought to be most important for neuron excitability. With the use of in vivo RNAi in adult bugs we found that α1C kno

SUBMITTER: Lohr JN 

PROVIDER: S-EPMC5732401 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature