GUCY2C maintains intestinal LGR5⁺ stem cells by opposing ER stress.

ABSTRACT: Long-lived multipotent stem cells (ISCs) at the base of intestinal crypts adjust their phenotypes to accommodate normal maintenance and post-injury regeneration of the epithelium. Their long life, lineage plasticity, and proliferative potential underlie the necessity for tight homeostatic regulation of the ISC compartment. In that context, the guanylate cyclase C (GUCY2C) receptor and its paracrine ligands regulate intestinal epithelial homeostasis, including proliferation, lineage commitment, and DNA damage repair. However, a role for this axis in maintaining ISCs remains unknown. Transgenic mice enabling analysis of ISCs (Lgr5-GFP) in the context of GUCY2C elimination (Gucy2c^-/- ) were combined with immunodetection techniques and pharmacological treatments

SUBMITTER: Kraft CL 

PROVIDER: S-EPMC5732700 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature