Allospecific Memory B Cell Responses Are Dependent on Autophagy.

ABSTRACT: Long-lived, donor-reactive memory B cells (Bmems) can produce alloantibodies that mediate transplant injury. Autophagy, an intrinsic mechanism of cell organelle/component recycling, is required for Bmem survival in infectious and model antigen systems, but whether autophagy affects alloreactive Bmem is unknown. We studied mice with an inducible yellow fluorescent protein (YFP) reporter expressed under the activation-induced cytidine deaminase (AID) promoter active in B cells undergoing germinal center reactions. Up to 12 months after allogeneic sensitization, splenic YFP⁺ B cells were predominantly IgD^- IgM^- IgG⁺ and expressed CD73, CD80, and PD-L2, consistent with Bmems. Labeled cells contained significantly more cells with autophagosomes and mo

SUBMITTER: Fribourg M 

PROVIDER: S-EPMC5732898 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature