Analysis of abrB Expression during the Infectious Cycle of Bacillus thuringiensis Reveals Population Heterogeneity.

ABSTRACT: Using the model host/pathogen pair Galleria mellonella/Bacillus thuringiensis, we have shown that these bacteria could kill their insect host, survive in its cadaver and form spores by sequentially activating virulence, necrotrophism and sporulation genes. However, the population isolated from the cadavers was heterogeneous, including non-sporulating cells in an unknown physiological state. To characterize these bacteria, we used a transcriptional fusion between the promoter of a gene expressed during early exponential growth (abrB) and a reporter gene encoding a destabilized version of GFP, in combination with a fluorescent reporter of the necrotrophic state. The composition of the bacterial population during infection was then analyzed by flow cytometry. We showed th

SUBMITTER: Ben Rejeb S 

PROVIDER: S-EPMC5732988 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature