Orchestrated positioning of post-transcriptional modifications at the branch point recognition region of U2 snRNA.

ABSTRACT: The branch point recognition region of spliceosomal snRNA U2 is heavily modified post-transcriptionally in most eukaryotic species. We focused on this region to learn how nearby positions may interfere with each other when targeted for modification. Using an in vivo yeast Saccharomyces cerevisiae cell system, we tested the modification activity of several guide RNAs from human, mouse, the frog Xenopus tropicalis, the fruit fly Drosophila melanogaster, and the worm Caenorhabditis elegans We experimentally verified predictions for vertebrate U2 modification guide RNAs SCARNA4 and SCARNA15, and identified a C. elegans ortholog of SCARNA15. We observed crosstalk between sites in the heavily modified regions, such that modification at one site may inhibit modi

SUBMITTER: Deryusheva S 

PROVIDER: S-EPMC5733568 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature