Local energetic frustration affects the dependence of green fluorescent protein folding on the chaperonin GroEL.

ABSTRACT: The GroE chaperonin system in Escherichia coli comprises GroEL and GroES and facilitates ATP-dependent protein folding in vivo and in vitro Proteins with very similar sequences and structures can differ in their dependence on GroEL for efficient folding. One potential but unverified source for GroEL dependence is frustration, wherein not all interactions in the native state are optimized energetically, thereby potentiating slow folding and misfolding. Here, we chose enhanced green fluorescent protein as a model system and subjected it to random mutagenesis, followed by screening for variants whose in vivo folding displays increased or decreased GroEL dependence. We confirmed the altered GroEL dependence of these variants with in vitro folding assays. Stri

SUBMITTER: Bandyopadhyay B 

PROVIDER: S-EPMC5733594 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature