Epigenetic modification of miR-663 controls mitochondria-to-nucleus retrograde signaling and tumor progression.

ABSTRACT: The normal cellular function requires communication between mitochondria and the nucleus, termed mitochondria-to-nucleus retrograde signaling. Disruption of this mechanism has been implicated in the development of cancers. Many proteins are known modulators of retrograde signaling, but whether microRNAs (miRNAs) are also involved is unknown. We conducted an miRNA microarray analysis using RNA from a parental cell line, a Rho⁰ line lacking mitochondrial DNA (mtDNA) and a Rho⁰ line with restored mtDNA. We found that miR-663 was down-regulated in the mtDNA-depleted Rho⁰ line. mtDNA restoration reversed this miRNA to parental level, suggesting that miR-663 may be epigenetically regulated by retrograde signaling. By using methylation-specific PCR and bisulfite s

SUBMITTER: Carden T 

PROVIDER: S-EPMC5733605 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature