Scalable Lentiviral Vector Production Using Stable HEK293SF Producer Cell Lines.

ABSTRACT: Lentiviral vectors (LV) represent a key tool for gene and cell therapy applications. The production of these vectors in sufficient quantities for clinical applications remains a hurdle, prompting the field toward developing suspension processes that are conducive to large-scale production. This study describes a LV production strategy using a stable inducible producer cell line. The HEK293 cell line employed grows in suspension, thus offering direct scalability, and produces a green fluorescent protein (GFP)-expressing lentiviral vector in the 10⁶ transduction units (TU)/mL range without optimization. The stable producer cell line, called clone 92, was derived by stable transfection from a packaging cell line with a plasmid encoding the transgene GFP. The packaging cell line exp

SUBMITTER: Manceur AP 

PROVIDER: S-EPMC5734158 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature