The metagenome of Caracolus marginella gut microbiome using culture independent approaches and shotgun sequencing.

ABSTRACT: Studies underestimate the microbial diversity and genotypic traits in the snails' microbiome. Caracolus marginella, a land snail native to Caribbean islands, can adapt to different environments. Our research focused on the generation of a metagenomic library from C. marginella gut, to further explore the diversity and functional traits. Thirty specimens of C. marginella were collected from the four regions of Puerto Rico. High molecular weight (40 kb) metagenomic libraries were generated using a direct DNA isolation method. DNA was end-repaired and ligated into a pCCFOS1 fosmid vector; then, the cloned DNA was transduced into Escherichia coli EPI300. The master pool library contains approximately 60,200 clones and restriction enzyme digestion showed that 90% of

SUBMITTER: Rabelo-Fernandez RJ 

PROVIDER: S-EPMC5734700 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature