Histamine Receptor 3 negatively regulates oligodendrocyte differentiation and remyelination.

ABSTRACT:

Background

Agents promoting oligodendrocyte precursor cell differentiation have the potential to restore halted and/or delayed remyelination in patients with multiple sclerosis. However, few therapeutic targets have been identified. The objective of this study was to identify novel targets for promotion of remyelination and characterize their activity in vitro and in vivo.

Methods

A high-content screening assay with differentiation of primary rat oligodendrocyte precursor cells was used to screen GSK-proprietary annotated libraries for remyelination-promoting compounds. Compounds were further validated in vitro and in vivo models; clinical relevance of target was confirmed in human post-mortem brain sections from patients with MS.

Results

Of ~1000 compounds screened,

SUBMITTER: Chen Y 

PROVIDER: S-EPMC5734789 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature