Postsynaptic synaptotagmins mediate AMPA receptor exocytosis during LTP.

ABSTRACT: Strengthening of synaptic connections by NMDA (N-methyl-d-aspartate) receptor-dependent long-term potentiation (LTP) shapes neural circuits and mediates learning and memory. During the induction of NMDA-receptor-dependent LTP, Ca²⁺ influx stimulates recruitment of synaptic AMPA (α-amino-3-hydroxy-5-methyl-4-isoxazole propionic acid) receptors, thereby strengthening synapses. How Ca²⁺ induces the recruitment of AMPA receptors remains unclear. Here we show that, in the pyramidal neurons of the hippocampal CA1 region in mice, blocking postsynaptic expression of both synaptotagmin-1 (Syt1) and synaptotagmin-7 (Syt7), but not of either alone, abolished LTP. LTP was restored by expression of wild-type Syt7 but not of a Ca²⁺-binding-deficient mutant Syt7. Blocking

SUBMITTER: Wu D 

PROVIDER: S-EPMC5734942 | biostudies-literature | 2017 Apr

REPOSITORIES: biostudies-literature