Prevalence of Trichinella spp. Infections in Hunted Wild Boars in Northern Iran.

ABSTRACT:

Background

Trichinellosis is an important and neglected foodborne zoonotic infectious disease in worldwide. The most human outbreaks in recent years have been related to consumption of wild boar meat. This cross-sectional study determined the prevalence of Trichinella spp. infections in hunted wild boars in northern Iran.

Methods

Thirty-five hunted wild boars were subjected in this study in 2015. All samples were examined by conventional artificial digestion method to detect of muscle larvae. Genomic DNA was extracted by phenol-chloroform method from isolated larvae. To identify the Trichinella species, a PCR-based method was applied using the internal transcribed spacer 2 (ITS2) and mitochondrial small-subunit ribosomal RNA (rRNA) gene sequences.

Results

SUBMITTER: Rostami A 

PROVIDER: S-EPMC5734972 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature