Refinement of the subunit interaction network within the nucleosome remodelling and deacetylase (NuRD) complex.

ABSTRACT: The nucleosome remodelling and deacetylase (NuRD) complex is essential for the development of complex animals. NuRD has roles in regulating gene expression and repairing damaged DNA. The complex comprises at least six proteins with two or more paralogues of each protein routinely identified when the complex is purified from cell extracts. To understand the structure and function of NuRD, a map of direct subunit interactions is needed. Dozens of published studies have attempted to define direct inter-subunit connectivities. We propose that conclusions reported in many such studies are in fact ambiguous for one of several reasons. First, the expression of many NuRD subunits in bacteria is unlikely to lead to folded, active protein. Second, interaction studies carried out in cells that contai

SUBMITTER: Torrado M 

PROVIDER: S-EPMC5734987 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature