Towards a defined ECM and small molecule based monolayer culture system for the expansion of mouse and human intestinal stem cells.

ABSTRACT: Current ISC culture systems face significant challenges such as animal-derived or undefined matrix compositions, batch-to-batch variability (e.g. Matrigel-based organoid culture), and complexity of assaying cell aggregates such as organoids which renders the research and clinical translation of ISCs challenging. Here, through screening for suitable ECM components, we report a defined, collagen based monolayer culture system that supports the growth of mouse and human intestinal epithelial cells (IECs) enriched for an Lgr5+ population comparable or higher to the levels found in a standard Matrigel-based organoid culture. The system, referred to as the Bolstering Lgr5 Transformational (BLT) Sandwich culture, comprises a collagen IV-coated porous substrate and a collagen I gel overlay which s

SUBMITTER: Tong Z 

PROVIDER: S-EPMC5735007 | biostudies-literature | 2018 Feb

REPOSITORIES: biostudies-literature