Longitudinal intravital imaging of the femoral bone marrow reveals plasticity within marrow vasculature.

ABSTRACT: The bone marrow is a central organ of the immune system, which hosts complex interactions of bone and immune compartments critical for hematopoiesis, immunological memory, and bone regeneration. Although these processes take place over months, most existing imaging techniques allow us to follow snapshots of only a few hours, at subcellular resolution. Here, we develop a microendoscopic multi-photon imaging approach called LIMB (longitudinal intravital imaging of the bone marrow) to analyze cellular dynamics within the deep marrow. The approach consists of a biocompatible plate surgically fixated to the mouse femur containing a gradient refractive index lens. This microendoscope allows highly resolved imaging, repeatedly at the same regions within marrow tissue, over months. LIMB reveals ex

SUBMITTER: Reismann D 

PROVIDER: S-EPMC5735140 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature