Hepatoma-Derived Growth Factor Secreted from Mesenchymal Stem Cells Reduces Myocardial Ischemia-Reperfusion Injury.

ABSTRACT:

Objectives

The present study aimed to explore the major factors that account for the beneficial effects of mesenchymal stem cells (MSCs).

Methods

Using isobaric tags for relative and absolute quantitation method, hepatoma-derived growth factor (HDGF) was identified as an important factor secreted by MSCs, but not by cardiac fibroblasts (CFs). The protective effects of conditioned medium (CdM) from MSCs or CFs were tested by using either H9C2 cells that were exposed by hypoxia-reoxygenation (H/R) insult or an in vivo mouse model of myocardial ischemia-reperfusion.

Results

Compared to CF-CdM, MSC-CdM conferred protection against reperfusion injury. CdM obtained from MSCs that were treated with HDGF-targeted shRNA failed to offer any protection in vitro. In

SUBMITTER: Zhou Y 

PROVIDER: S-EPMC5735317 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature