Proteomics analysis and proteogenomic characterization of different physiopathological human lenses.

ABSTRACT:

Background

The aim of the present study was to identify the proteomic differences among human lenses in different physiopathological states and to screen for susceptibility genes/proteins via proteogenomic characterization.

Methods

The total proteomes identified across the regenerative lens with secondary cataract (RLSC), congenital cataract (CC) and age-related cataract (ARC) groups were compared to those of normal lenses using isobaric tagging for relative and absolute protein quantification (iTRAQ). The up-regulated proteins between the groups were subjected to biological analysis. Whole exome sequencing (WES) was performed to detect genetic variations.

Results

The most complete human lens proteome to date, which consisted of 1251 proteins, including 55.2% previous

SUBMITTER: Wu X 

PROVIDER: S-EPMC5735820 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature