Progranulin derivative Atsttrin protects against early osteoarthritis in mouse and rat models.

ABSTRACT:

Background

Atsttrin, an engineered protein composed of three tumor necrosis factor receptor (TNFR)-binding fragments of progranulin (PGRN), shows therapeutic effect in multiple murine models of inflammatory arthritis . Additionally, intra-articular delivery of PGRN protects against osteoarthritis (OA) progression. The purpose of this study is to determine whether Atsttrin also has therapeutic effects in OA and the molecular mechanisms involved.

Methods

Surgically induced and noninvasive rupture OA models were established in mouse and rat, respectively. Cartilage degradation and OA were evaluated using Safranin O staining, immunohistochemistry, and ELISA. Additionally, expressions of pain-related markers, degenerative factors, and anabolic and catabolic markers known to be in

SUBMITTER: Wei JL 

PROVIDER: S-EPMC5735869 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature