A tissue-based approach to selection of reference genes for quantitative real-time PCR in a sheep osteoporosis model.

ABSTRACT:

Background

In order to better understand the multifactorial nature of osteoporosis, animal models are utilized and compared to healthy controls. Female sheep are well established as a model for osteoporosis induced by ovariectomy, calcium and vitamin D low diet, application of steroids, or a combination of these treatments. Transcriptional studies can be performed by applying quantitative real time PCR (RT-qPCR). RT-qPCR estimates mRNA-levels of target genes in relation to reference genes. A chosen set of reference genes should not show variation under experimental conditions. Currently, no standard reference genes are accepted for all tissue types and experimental conditions. Studies examining reference genes for sheep are rare and only one study described stable reference in mand

SUBMITTER: Schulze F 

PROVIDER: S-EPMC5735898 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature