ATNT: an enhanced system for expression of polycistronic secondary metabolite gene clusters in Aspergillus niger.

ABSTRACT:

Background

Fungi are treasure chests for yet unexplored natural products. However, exploitation of their real potential remains difficult as a significant proportion of biosynthetic gene clusters appears silent under standard laboratory conditions. Therefore, elucidation of novel products requires gene activation or heterologous expression. For heterologous gene expression, we previously developed an expression platform in Aspergillus niger that is based on the transcriptional regulator TerR and its target promoter PterA.

Results

In this study, we extended this system by regulating expression of terR by the doxycycline inducible Tet-on system. Reporter genes cloned under the control of the target promoter PterA remained silent in the absence of do

SUBMITTER: Geib E 

PROVIDER: S-EPMC5735947 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature