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Low-input Capture-C: A Chromosome Conformation Capture Assay to Analyze Chromatin Architecture in Small Numbers of Cells.

ABSTRACT: Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This protocol describes two new low-input Capture-C approaches that can generate high-quality 3C interaction profiles from 10,000-20,000 cells, depending on the resolution used for analysis.

SUBMITTER: Oudelaar AM

PROVIDER: S-EPMC5736099 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature

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Low-input Capture-C: A Chromosome Conformation Capture Assay to Analyze Chromatin Architecture in Small Numbers of Cells.

Oudelaar A Marieke AM Downes Damien J DJ Davies James O J JOJ Hughes Jim R JR

Bio-protocol 20171201 23

Chromosome conformation capture (3C) techniques are crucial to understanding tissue-specific regulation of gene expression, but current methods generally require large numbers of cells. This protocol describes two new low-input Capture-C approaches that can generate high-quality 3C interaction profiles from 10,000-20,000 cells, depending on the resolution used for analysis. ...[more]

PMID: 29270442

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