Proteomics show antigen presentation processes in human immune cells after AS03-H5N1 vaccination.

ABSTRACT: Adjuvants enhance immunity elicited by vaccines through mechanisms that are poorly understood. Using a systems biology approach, we investigated temporal protein expression changes in five primary human immune cell populations: neutrophils, monocytes, natural killer cells, T cells, and B cells after administration of either an Adjuvant System 03 adjuvanted or unadjuvanted split-virus H5N1 influenza vaccine. Monocytes demonstrated the strongest differential signal between vaccine groups. On day 3 post-vaccination, several antigen presentation-related pathways, including MHC class I-mediated antigen processing and presentation, were enriched in monocytes and neutrophils and expression of HLA class I proteins was increased in the Adjuvant System 03 group. We identified several protein familie

SUBMITTER: Galassie AC 

PROVIDER: S-EPMC5736144 | biostudies-literature | 2017 Jun

REPOSITORIES: biostudies-literature