Comparative study of impaction and sedimentation in an aerosol chamber using defined fungal spore and bacterial concentrations.

ABSTRACT: Biocontamination control is a very significant part of the manufacturing process of sterile drugs. Sterility is frequently monitored by active or passive air sampling measurements, but there are no specific rules as to how this is to be done. This study tested air sampling methods of active impaction and passive sedimentation under standardized conditions. Aspergillus niger (A. niger) and Staphylococcus aureus (S. aureus) were selected in this experiment to examine parallels, correlations and differences between the two methods. The results show that the number of colony forming units per plate (CFU/plate) was higher for A. niger in the active method, whereas for S. aureus it was higher in the sedimentation method. A high correlation coefficient was found between the impaction and sediment

SUBMITTER: Haas D 

PROVIDER: S-EPMC5736173 | biostudies-literature | 2017

REPOSITORIES: biostudies-literature