Micro- and Nanoscale Topographies on Silk Regulate Gene Expression of Human Corneal Epithelial Cells.

ABSTRACT:

Purpose

Corneal basement membrane has topographical features that provide biophysical cues to direct cell adherence, migration, and proliferation. In this study, we hypothesize that varying topographic pitch created on silk films can alter epithelial cell morphology, adhesion, and the genetic expression involved in cytoskeletal dynamics-related pathways.

Methods

Silicon wafers with parallel ridge widths of 2000, 1000, and 800 nm were produced and used to pattern silk films via soft lithography. Human corneal epithelial cells were cultured onto silk. After 72 hours of incubation, images were taken to study cell morphology and alignment. Cytoskeletal structures were studied by immunofluorescent staining. RNA was collected from cultured cells to perform RNA-Seq transcriptome an

SUBMITTER: Kang KB 

PROVIDER: S-EPMC5736325 | biostudies-literature | 2017 Dec

REPOSITORIES: biostudies-literature