Proteomic analysis reveals that a global response is induced by subinhibitory concentrations of ampicillin.

ABSTRACT: In this study, a recipient-donor co-culture system was used to research the effect of subinhibitory concentrations of antibiotics on horizontal transmission in bacteria and the influence of antibiotics on protein expression. We employed two-dimensional gel electrophoresis combined with mass spectrometry to compare the protein expression profiles in systems with or without 0.5 × the minimum inhibitory concentration of ampicillin. RT-PCR was used to assess the transcriptional levels of the differentially expressed genes. Fifty-seven different proteins were induced or suppressed. The upregulated proteins were involved in transcription and translation, cell wall synthesis, bacterial SOS response, and detoxifying functions, and the downregulated proteins were involved in metabolism. These resul

SUBMITTER: Xiong L 

PROVIDER: S-EPMC5736336 | biostudies-literature | 2017 Nov

REPOSITORIES: biostudies-literature