Detection of 224 candidate structured RNAs by comparative analysis of specific subsets of intergenic regions.

ABSTRACT: The discovery of structured non-coding RNAs (ncRNAs) in bacteria can reveal new facets of biology and biochemistry. Comparative genomics analyses executed by powerful computer algorithms have successfully been used to uncover many novel bacterial ncRNA classes in recent years. However, this general search strategy favors the discovery of more common ncRNA classes, whereas progressively rarer classes are correspondingly more difficult to identify. In the current study, we confront this problem by devising several methods to select subsets of intergenic regions that can concentrate these rare RNA classes, thereby increasing the probability that comparative sequence analysis approaches will reveal their existence. By implementing these methods, we discovered 224 novel ncRNA classes, which inc

SUBMITTER: Weinberg Z 

PROVIDER: S-EPMC5737381 | biostudies-literature | 2017 Oct

REPOSITORIES: biostudies-literature