A data-driven structural model of hSSB1 (NABP2/OBFC2B) self-oligomerization.

ABSTRACT: The maintenance of genome stability depends on the ability of the cell to repair DNA efficiently. Single-stranded DNA binding proteins (SSBs) play an important role in DNA processing events such as replication, recombination and repair. While the role of human single-stranded DNA binding protein 1 (hSSB1/NABP2/OBFC2B) in the repair of double-stranded breaks has been well established, we have recently shown that it is also essential for the base excision repair (BER) pathway following oxidative DNA damage. However, unlike in DSB repair, the formation of stable hSSB1 oligomers under oxidizing conditions is an important prerequisite for its proper function in BER. In this study, we have used solution-state NMR in combination with biophysical and functional experiments to obtain a structural m

SUBMITTER: Touma C 

PROVIDER: S-EPMC5737504 | biostudies-literature | 2017 Aug

REPOSITORIES: biostudies-literature