Analysis of primary microRNA loci from nascent transcriptomes reveals regulatory domains governed by chromatin architecture.

ABSTRACT: Changes in mature microRNA (miRNA) levels that occur downstream of signaling cascades play an important role during human development and disease. However, the regulation of primary microRNA (pri-miRNA) genes remains to be dissected in detail. To address this, we followed a data-driven approach and developed a transcript identification, validation and quantification pipeline for characterizing the regulatory domains of pri-miRNAs. Integration of 92 nascent transcriptomes and multilevel data from cells arising from ecto-, endo- and mesoderm lineages reveals cell type-specific expression patterns, allows fine-resolution mapping of transcription start sites (TSS) and identification of candidate regulatory regions. We show that inter- and intragenic pri-miRNA transcripts span vast genomic regi

SUBMITTER: Bouvy-Liivrand M 

PROVIDER: S-EPMC5737680 | biostudies-literature | 2017 Sep

REPOSITORIES: biostudies-literature